KMID : 0380019940090050492
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Korean Journal of Biotechnology and Bioengineering 1994 Volume.9 No. 5 p.492 ~ p.498
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Hollow Fiber Dialysis Culture of E. coli
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Abstract
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A hollow fiber device was utilized to perform a dialysis culture for E. colt. Acetic acid inhibition on the growth of E. colt was relieved by dialyzing the acid from broth into a dialysate reservoir. The rate of acetic acid formation was very sensitive to the concentration of glucose and dissolved oxygen. There¡©fore it was found that the glucose permeation rate should be balanced with the oxygen supply rate. Spe¡©cific growth rate of E. colt¢¥ was determined by the glucose permeation rate through membrane. Under a low permeation rate, acetic acid formation was depressed in accordance with high dissolved oxygen concentration as well as low glucose concentration.
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